Effects of Biological Materials and Collection Media on PCR Detection of <i>Tritrichomonas foetus</i>
Effects of Biological Materials and Collection Media on PCR Detection of <i>Tritrichomonas foetus</i>作者机构：California Animal Health and Food Safety Laboratory System Davis CA USA Department of Population Health and Reproduction School of Veterinary Medicine University of California Davis Davis CA USA Great Plains Veterinary Educational Center University of Nebraska-Lincoln Clay Center NE USA
出 版 物：《Open Journal of Animal Sciences》 (动物科学期刊（英文）)
年 卷 期：2019年第9卷第1期
页 面：121-128页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Tritrichomonas foetus PCR Detection Urine Semen Blood
摘 要：Tritrichomonas foetus is an important pathogen of the bovine reproductive tract causing early embryonic death and abortion in cows and persistent, asymptomatic infection in bulls. PCR detection methods have greatly enhanced diagnostic accuracy over culture;however, pre-analytical sample handling is just as critical as technical performance in detecting this pathogen and is not well studied. The purpose of this study was to investigate the effects of biological materials present in the prepuce on PCR detection of T. foetus in a variety of collection media. Simulated preputial samples were created using InPouchTM (IP) media, lactated ringers solution (LRS), or sterile saline (SAL);inoculated with low numbers of one of three T. foetus strains;and spiked with either blood, semen, urine, or sham treatment. Samples were transported to the lab, placed in growth media (LRS and SAL samples), incubated, and tested for T. foetus by PCR. Samples containing urine had statistically significantly greater mean Ct values (P = 0.008) than samples containing other materials, seen most dramatically in IP (P 0.0001.) Urine contamination resulted in significantly (P = 0.037) fewer samples being identified as “positive for T. foetus. Overall, SAL collections also had significantly higher mean Ct than IP or LRS (P 0.001), and were less likely (P = 0.018) to results in classification as a “positive sample. Results of this study indicate that collection media and biological materials can affect T. foetus PCR detection. The presence of urine in preputial samples can result in false negative results, while blood had no detrimental effects.
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