Sensitivity assay of polymerase chain reaction for detection of Canine Parvo Virus infection in dogs
Sensitivity assay of polymerase chain reaction for detection of Canine Parvo Virus infection in dogs作者机构：Central Military Veterinary Laboratory Meerut Uttarpradesh India
出 版 物：《Open Journal of Clinical Diagnostics》 (临床诊断学期刊（英文）)
年 卷 期：2012年第2卷第3期
页 面：45-47页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：CPV Canine Parvo Virus PCR Polymerase Chain Reaction Sensitivity
摘 要：A polymerase chain reaction was performed using re-ported primers for detection of Canine Parvo virus (CPV) in the stool sample obtained from repository. The PCR primers were specific to VP1/VP2 gene of CPV. Sensi-tivity assay of PCR detection was performed by making dilutions of CPV positive DNA extracted from fecal sample, carrying out PCR for each dilution and visualiz-ing amplicons in ethidium bromide stained agarose gel under UV radiation. Study was valuable in determining the efficiency of PCR. The sensitivity of PCR in present study was determined to be equivalent to detection of .00 2pg/μl of CPV DNA. The study was conducted to analyze the variation, sensitivity and repeatability.
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