Analysis of Enterobacteriaceae Producing Broad-Spectrum Beta-Lactamases in the Intensive Care Unit Setting
在重症监护病房设置生产广谱β-内酰胺酶肠杆菌科分析作者机构：Department of MicrobiologyFaculty of Medicine and DentistryPalacky University OlomoucOlomoucCzech Republic University Hospital OlomoucOlomoucCzech Republic Department of Preventive MedicineFaculty of Medicine and DentistryPalacky University OlomoucOlomoucCzech Republic
出 版 物：《Open Journal of Medical Microbiology》 (医学微生物学（英文）)
年 卷 期：2013年第3卷第1期
页 面：56-61页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：Supported by the following grant projects:LF_2012_006 and MSM6198959223
主 题：Enterobacteriaceae Broad-Spectrum Beta-Lactamases Intensive Care Setting
摘 要：Strains of the Enterobacteriaceae family producing ESBL and AmpC broad-spectrum beta-lactamases that may survive in the hospital setting potentially cause infection in hospitalized patients due to contaminated objects or health care workers’ hands. Over a period of two months (November-December 2010), a single epidemiological study of microbial contamination of air, surfaces and health care workers (swabs from both nostrils and the right hand without a glove) was carried out at two intensive care units of the University Hospital Olomouc, Czech Republic. The bacteria were identified using standard microbiological methods. Phenotypic detection of ESBL and AmpC enzymes and basic genetic analysis of ESBL- and AmpC-positive isolates was performed. The same approach was used to identify and analyze bacteria isolated from clinical samples of patients hospitalized at the above departments over the study period. From a total of 140 environmental samples collected over the study period, 21 isolates of the Enterobacteriaceae family were identified, with ESBL and AmpC production being detected in 4 and 7 isolates, respectively. Among patients’ clinical samples, 10 ESBL- and 6 AmpC-positive isolates were detected. No similarity was found between environmental isolates and strains isolated from patients.
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