Experimental Validation and Simulation of Fourier and Non-Fourier Heat Transfer Equation during Laser Nano-Phototherapy of Lung Cancer Cells: An <i>in Vitro</i>Assay

作     者：Mohammad E. Khosroshahi Lida Ghazanfari Payam Khoshkenar 

作者机构：Center for Advanced Diffusion-Wave Technologies (CADIFT) Department of Mechanical and Industrial Engineering University of Toronto Toronto Canada Laser and Nanobiophotonics Laboratory Biomaterial Group Faculty of Biomedical Engineering Amirkabir University of Technology Tehran Iran Max Planck Institute for the Science of Light Erlangen Germany Department of Biomedical Engineering College of Engineering & Science Louisiana Technology University Ruston USA 

出 版 物：《Journal of Modern Physics》 (现代物理（英文）)

年 卷 期：2014年第5卷第18期

页      面：2125-2141页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Bioheat Equation Gold Nanoshells Laser Hyperthermia Lung Cancer Cells Surface Plasmon Resonance 

摘      要：This paper investigated the numerical scheme extended to solve the hyperbolic non-Fourier form of bioheat transfer equation and the experimental trials were conducted to validate the numerical simulation. MNPs were prepared via co-precipitation and modified with a silica layer. The amino modified Fe3O4/SiO2 nanoshells were covered with gold colloids producing nanoshells of Fe3O4/SiO2/Au (MNSs). In vitro assays were performed to determine the effect of apoptosis of QU-DB lung cancer cells based on the cells morphology changes. Cell damage was reduced by decreasing the power density of laser. Also, a larger area of damage on cell culture plates was observed at longer intervals of laser irradiation. The effect of nanoshell concentration and irradiation rate has been evaluated. A maximum temperature rise of 6°C was achieved at 184 W/cm2 and concentration of 0.01 mg/ml. The experiment confirmed a hyperbolic behaviour of thermal propagation. The results revealed that the three-dimensional implementation of bioheat equation is likely to be more accurate than the two-dimensional study.