A simplified and miniaturized glucometer-based assay for the detection of β-glucosidase activity

作     者：Min-yi JIN Tong ZHANG Yi-shun YANG Yue DING Jun-song LI Gao-ren ZHONG 

作者机构：Experiment Center for Teaching and LearningShanghai University of Traditional Chinese MedicineShanghai 201203China School of PharmacyFudan UniversityShanghai 200032China 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2019年第20卷第3期

页      面：264-274页

核心收录：

学科分类：1010[医学-医学技术（可授医学、理学学位）] 10[医学] 

基　　金：Project supported by the National Natural Science Foundation of China(No.81173561) the Program of Shanghai Academic/Technology Research Leader(No.18XD1403700) China 

主　　题：Glucometer-based assay β-Glucosidase Activity detection 

摘      要：β-Glucosidase activity assays constitute an important indicator for the early diagnosis of neonatal necrotizing enterocolitis and qualitative changes in medicinal *** drawbacks of the existing methods are high consumption of both time and reagents,complexity in operation,and requirement of expensive instruments and highly trained *** present study provides a simplified,highly selective,and miniaturized glucometer-based strategy for the detection ofβ-glucosidase ***-factor experiments showed that optimumβ-glucosidase activity was exhibited at 50°C and pH 5.0 in a citric acid-sodium citrate buffer when reacting with 0.03 g/mL salicin for 30 *** procedure for detection was simplified without the need of a chromogenic *** of the analytical method demonstrated that the accuracy,precision,repeatability,stability,and durability were *** linear ranges ofβ-glucosidase in a buffer solution and rat serum were 0.0873–1.5498 U/mL and 0.4076–2.9019 U/mL,*** proposed method was free from interference fromβ-dextranase,snailase,β-galactosidase,hemicellulase,and glucuronic acid released by *** demonstrated that the proposed assay had a higher selectivity than the conventional dinitrosalicylic acid(DNS)assay because of the specificity for salicin and unique recognition of glucose by a personal glucose *** of the method resulted in a microassay forβ-glucosidase *** easy-to-operate method was successfully used to detect a series ofβ-glucosidases extracted from bitter almonds and cultured by Aspergillus *** addition,the simplified and miniaturized glucometer-based assay has potential application in the point-of-care testing ofβ-glucosidase in many fields,including medical diagnostics,food safety,and environmental monitoring.