<i>In Vitro</i>Germination and Plantlet Establishment of Wild Chamomile of Morocco <i>Cladanthus mixtus</i>(L.) Oberpr. and Vogt

作     者：Naima Harras Ahmed Lamarti 

作者机构：Laboratoire de Biotechnologie et d’Amélioration des Plantes Département de Biologie Faculté des Sciences M’Hannech II Tétouan Maroc 

出 版 物：《American Journal of Plant Sciences》 (美国植物学期刊（英文）)

年 卷 期：2014年第5卷第18期

页      面：2623-2632页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Asymbiotic Seed Germination In Vitro Propagation Basal Media Cladanthus mixtus (L.) 

摘      要：In vitro seeds germination and plantlets establishment of Cladanthus mixtus (L.) Oberpr. and Vogt were studied in this report. A reliable protocol was developed for in vitro seed germination, multiplication and plantlet regeneration of Cladanthus mixtus. The seeds were sterilized and cultured in different media. Among the two basal media evaluated for symbiotic seed germination, gelled distilled water was found to be the best with a high percentage of seeds germination (100%) after 33 hours of culture. Seedlings were further transferred to different types of media. Result observed after 4 weeks showed that MS medium promotes the highest growth with an average of 2.75 ± 0.12 cm shoot length and 2.60 ± 0.29 shoots per explants, and the mean number of roots achieved 3.33 ± 0.17 root per explants with a length of 2.42 ± 0.16 cm. This study showed that macroelements of MS (1962) medium is essential for in vitro shoot multiplication, growth and rooting of shoots of Cladanthus mixtus L.