<i>In Vitro </i>Effects of Oil’s Fatty Acids on T-Cell Function of Obese Men

作     者：Asmaa Meraou Hafida Merzouk Amel Saidi Amel Medjdoub Sid Ahmed Merzouk Mohamed Bénali Slimane Belbraouet 

作者机构：Laboratory of Physiology Physiopathology and Biochemistry of Nutrition Department of Biology Faculty of Natural and Life Sciences Earth and Universe University of Abou-Bekr Belkaï d Tlemcen Algeria Laboratory of Biotoxicology Djilali Liabes University Sidi Bel Abbès Algeria Department of Technical Sciences Faculty of Engineering University of Abou-Bekr Belkaï d Tlemcen Algeria école de Nutrition Université de Moncton New Brunswick Canada 

出 版 物：《Food and Nutrition Sciences》 (食品与营养科学（英文）)

年 卷 期：2017年第8卷第2期

页      面：277-293页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Obesity Lymphocytes Olive Oil Linseed Oil Nigel Oil Sunflower Oil Cytokines Oxidant/Antioxidant Status 

摘      要：Background: Dietary fatty acids have important homeostatic functions in regulating the immune response and may exert beneficial effects on immune alterations during obesity. Objective: To assess the in vitro effects of oil fatty acids, different oils (olive, linseed, Nigel, sunflower) were tested on T-lymphocyte proliferation, Th1- and Th2-type cytokine production, and intracellular oxidant/antioxidant status in obese patients. Methods: Peripheral blood lymphocytes were isolated using Histopaque and were in vitro cultured and stimulated by Con A in the presence or absence of the oils. Cell proliferation, interleukin-2, interleukin-4 and interferon-γ (INFγ) secretions and intracellular oxidative status (glutathione (GSH), malondialdehyde (MDA), carbonyl protein levels, catalase activity and micronuclei frequency) were investigated. Results: Abnormalities in lymphocyte function and intracellular oxidative stress were observed in obesity. Linseed oil induced a reduction in T-lymphocyte proliferation and IL-2 production while Nigel oil increased them in both obese and control groups. In addition, Nigel oil enhanced IFNγ and IL-4 secretion. Olive and sunflower oils had no effect on lymphocyte proliferation and cytokine secretion in both groups. Linseed and Nigel oils induced an increase in T cell GSH concentrations and catalase activity with a concomitant decrease in MDA, carbonyl protein contents and micronuclei frequency especially in obese patients. Conclusion: Linseed and Nigel oils had beneficial effects on lymphocyte proliferation, cytokines secretions and redox status, while olive and sunflower oils had no effects on immune cell function in obesity.