In Vitro Evaluation of Gamma Irradiation on a Gel Formulation of Cratylia Mollis: Rheological Proporties and Microbiological Control

作     者：Maria Helena Madruga Lima-Ribeiro Ralph Santos-Oliveira Mauricelia Firmino De Santana Terezinha De Jesus Andreoli Pinto Irene Satiko Kikuchi Cheila Goncalves Mothe Luana Cassandra Breitenbach Barroso Coelho Maria Tereza Dos Santos Correia Ana Maria Dos Anjos Carneiro-Leao 

作者机构：Laboratory of Immunopathology Keizo Asami(LIKA)Federal University of PernambucoRecifeBrazil Nuclear Engineering InstituteLaboratory of NanoradiopharmaceuticalsRio de JaneiroBrazil Biochemistry DepartmentFederal University of PernambucoRecifeBrazil Pharmacy DepartmentUniversity of Sao PauloSao PauloBrazil School of ChemistryFederal University of Rio de JaneiroRio de JaneiroBrazil Departament of Morphology and Animal PhysiologyFederal Rural University of PernambucoRecifeBrazil 

出 版 物：《Journal of Cosmetics, Dermatological Sciences and Applications》 (化妆品、皮肤病及应用期刊（英文）)

年 卷 期：2012年第2卷第2期

页      面：45-50页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：The Department of Energy’s Nuclear UFPE/FINEP the Nuclear Energy Institute in Rio de Janeiro 

主　　题：Gamma Radiation Natural Products Hemagglutinating Activity Cramoll-1 4 

摘      要：Lectin Cramoll-1,4, obtained from Cratylia mollis seeds (beans camaratu) was structurally characterized, biologically and pharmacologically, but its use as a biopharmaceutical is not well documented. The objective of this study is to propose a biopharmaceutical formulation lectin Cramoll-1,4, test their hemagglutinating properties in vitro as well as the use of gamma radiation as a continuous process of decontamination formulation. It was made of the extraction and purification Cramoll-1,4, was developed a gel formulation using Carbopol? as a vehicle, at concentrations of 50, 100, 200, 300 and 600 μg was irradiated with 60Co gamma rays in a dose of 7.549 kGy·h–1. The proposed formulation at a concentration of 300 μg produced an increase in the hemagglutinating units Cramoll-1,4 due to the synergistic effect caused by gamma radiation. Considering the diverse use of lectins, specific molecular and structural factors, as well as changes resulting from its formulation, concentration, irradiation and route of administration is of utmost importance to continue the studies in vitro, for subsequent application in vivo to characterize the physiological and molecular processes involved in the response and cellular effects.