Human Beta Defensins 1,2 and 3 Produced by Amniochorion Membranes Is Similar in Term and Preterm Delivery

作     者：Nathália Mayumi Noda-Nicolau Jossimara Polettini Camila Marconi José Carlos Peracoli Hélio Amante Miot Márcia Guimaraes da Silva 

作者机构：Department of Pathology Botucatu Medical School UNESP—Universidade Estadual Paulista Sao Paulo Brazil The University of Western Sao Paulo (UNOESTE) Presidente Prudente Brazil Department of Basic Pathology Setor de Ciências Biológicas UFPR—Universidade Federal do Paraná Curitiba Brazil Department of Gynecology and Obstetrics Botucatu Medical School UNESP—Universidade Estadual Paulista Sao Paulo Brazil Department of Dermatology Botucatu Medical School UNESP—Universidade Estadual Paulista Sao Paulo Brazil 

出 版 物：《Open Journal of Obstetrics and Gynecology》 (妇产科期刊（英文）)

年 卷 期：2017年第7卷第8期

页      面：846-857页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Preterm Birth Amniochorion Membranes Human Beta Defensins 

摘      要：Amniochorion membranes were collected from 25 pregnant women at preterm labor, in the presence or not of Preterm Premature Rupture of Membranes (PPROM) and 27 pregnant women at term in the presence at labor, in order to quantify the expression and to evaluate the immunoreactivity of human beta defensins (HBD)1, HBD2, HBD3 and HBD4 in amniochorion membranes from pregnancies complicated by spontaneous prematurity. The HBDs were evaluated by immunohistochemistry, real time quantitative PCR and ELISA. Statistical analyses were performed using Chi-squared and Mann Whitney tests. There was no significant difference in HBDs expression between study and control groups: HBD1 (Md = 0.62 (0.0 - 105.0) vs Md = 0.80 (0.02 - 25.0);p = 0.85), HBD2 (Md = 0.17 (0.0 - 5.2) vs Md = 0.0 (0.0 - 43.2);p = 0.16), HBD3 (Md = 0.11 (0.0 - 140.5) vs Md = 0.06 (0.0 - 972.1);p = 0.91). Also, HBD1, HBD2 and HBD3 protein expression was not significant different between the groups: HBD1 (1.32 pg/mL (0.0 - 1.85) vs 1.08 pg/mL (0.04 - 2.22);p = 0.67), HBD2 (0.00 pg/mL (0.0 - 1.74) vs 0.02 pg/mL (0.0 - 1.24);p = 0.69), HBD3 (0.04 pg/mL (0.0 - 1.05) vs 0.09 pg/mL (0.0 - 1.05);p = 0.63). The immunoreactivity of HBD1, HBD2 and HBD3 was observed in amnion, chorion and decidua cells from preterm and term pregnancies. Amniochorion membranes are sources of HBD1, HBD2 and HBD3 and their expressions are similar in term and preterm pregnancies.