Development of a Genetic Transformation Method for Seabuckthorn (<i>Hippophae rhamnoides</i>L.)

作     者：Sridevy Sriskandarajah David Clapham Per-Olof Lundquist 

作者机构：Department of Plant Biology Uppsala BioCenter Lin-nean Centre of Plant Biology in Uppsala Swedish University of Agricultural Sciences Uppsala Sweden 

出 版 物：《American Journal of Plant Sciences》 (美国植物学期刊（英文）)

年 卷 期：2014年第5卷第5期

页      面：528-534页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Hippophae rhamnoides Particle Bombardment Transformation 

摘      要：Seabuckthorn (Hippophae rhamnoides L.) is a dioecious plant with berries containing high amounts of several bioactive compounds with nutritional and medicinal traits. It is also planted to control soil erosion. A genetic transformation procedure will facilitate studies of the control of plant development and interactions with symbionts and pathogens, and will provide a tool for plant breeding. Here, we present a particle bombardment method for transforming seabuckthorn. The early stages of induced adventitious shoots from roots were chosen as a novel target tissue for the transformation procedure. The root system was bombarded with gold particles coated with plasmid pRT99gus containing genes for plant kanamycin resistance and for β-glucuronidase expression, and shoots were regenerated under kanamycin selection. PCR analysis of the regenerated transformed lines confirmed the presence of a 603 bp gus (uidA) gene fragment and a 1.5 kb fragment from the 35S promoter in three shoots from independent transformation events.