Mass production, fermentation, formulation of <i>Pseudomonas putida</i>for controlling of die back and phomopsis diseases on grapevine
Mass production, fermentation, formulation of <i>Pseudomonas putida</i>for controlling of die back and phomopsis diseases on grapevine作者机构：Biotechnology and Genetic Engineering Unit Dokki Egypt Pathology Department National Research Centre Dokki Egypt Pomology Department National Research Centre Dokki Egypt
出 版 物：《Advances in Bioscience and Biotechnology》 (生命科学与技术进展（英文）)
年 卷 期：2013年第4卷第6期
页 面：741-750页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Die Back Phomopsis Diseases Grapevine and Pseudomonas putida
摘 要：The export oriented agricultural and horticultural crops depends on the export of residue free produce and has created a great potential and demand for the incorporation of biopesticides in crop protection. PGPR with wide scope for commercialization includes Pseudomonas putida. Pseudomonas putida was effective in reducing die back caused by a fungus Botryodiplodia theobromae and phomopsis or arm death, caused by a fungus, Phomopsis viticola of grapevine in vitro and in vivo. Pseudomonas putida showed optimum Siderophore pseudobactin production at 72 h, and growth peak at 120 h. glycerol as a source of carbon and yeast as a source of nitrogen Pseudomonas putida was formulated using polysaccharide dextran starch, which led to the elongation of conservation and the effectiveness of the biofungicide. Pseudomonas putida was very effective as biocontrol agents to reduce the die back and phomopsis diseases on grapevine. The obtained results showed that both bacterial treatments significantly increased the growth parameters as well as fruits dry weights and yield of grapevine.
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