Adipocyte Derived Mesenchymal Stromal Cell and Platelet Lysate: Ideal Cell and Supplement for the Treatment of Immune-Inflammatory Diseases?
Adipocyte Derived Mesenchymal Stromal Cell and Platelet Lysate: Ideal Cell and Supplement for the Treatment of Immune-Inflammatory Diseases?作者机构：Cellular Therapy Center Hospital de Clínicas de Porto Alegre Porto Alegre Brazil Laboratory of Cell Culture and Molecular Analysis of Human Cells Hospital de Clínicas de Porto Alegre Porto Alegre Brazil Laboratory of Cell Culture and Molecular Analysis of Human Cells Hospital de Clínicas de Porto Alegre Porto Alegre Brazil Post-graduation in Medicine: Medical Sciences Federal University of Rio Grande do Sul Porto Alegre Brazil
出 版 物：《CellBio》 (细胞生物学（英文）)
年 卷 期：2016年第5卷第2期
页 面：15-25页
学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学]
主 题：Mesenchymal Stromal Cells Dental Pulp Adipose Tissue Bone Marrow T Cells Immunomodulatory Effect
摘 要：Background: Mesenchymal stromal cells (MSC) are being tested for the treatment of immune diseases. MSC are present in several adult tissues which milieu may influence MSC behavior particularly under inflammatory conditions. Additionally, culture conditions also can modify cell function or state of activation. Methods: To address the influence of the MSC source on its characteristics, we studied a xenofree, platelet lysate supplemented MSC from dental pulp, adipose tissue and bone marrow, co-cultured with isolated T cells and PBMC subset, and studied the effect of culture animal or human supplements immunomodulatory effect. Results: All three sources were efficient in inhibiting T cells. Among all MSC sources, as also described by others, adipose MSC was capable to significantly induce Treg phenotype and decrease T CD8+. Furthermore, comparing fetal bovine serum and platelet lysate, results demonstrate that platelet lysate alone is capable to induce immunomodulatory phenotype. Additional studies have to be made to elucidate the PL immunomodulatory effect.
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