Cannabinoids Production by Hairy Root Cultures of <i>Cannabis sativa</i>L.
Cannabinoids Production by Hairy Root Cultures of <i>Cannabis sativa</i>L.作者机构：Technische Biochemie Fachbereich Bio- und Chemieingenieurwesen Technische Universität Dortmund Dortmund Germany
出 版 物：《American Journal of Plant Sciences》 (美国植物学期刊（英文）)
年 卷 期：2015年第6卷第11期
页 面：1874-1884页
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
主 题：Tetrahydrocannabinol Cannabinoids Callus Hairy Root Cannabis sativa
摘 要：Tetrahydrocannabinol (THC) derivatives are used clinically as analgesic, anti-inflammatory, appetite stimulant, anti-emetic and anti-tumor cannabinoids. THC and its related compounds are at present obtained by extraction from intact Cannabis plants or chemical synthesis, but plant cell cultures may be an alternative source of production. In the present study, hairy root cultures of C. sativa (Cannabaceae) were induced by incubation of aseptically grown callus culture with solid B5 medium supplemented with 4 mg/l naphthaleneacetic acid in darkness at 25°C. Hairy root growth profiles in shake flask, increased periodically during 35 days of growth cycle. The cannabinoid contents produced in minor levels and remained below 2.0 μg/g dry weight. The contents of can-nabinoid were analyzed by liquid chromatography and confirmed by mass spectrometry.
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