Low-Density Co-Inoculation with Bradyrhizobium japonicum SAY3-7 and Streptomyces griseoflavus P4 Promotes Plant Growth and Nitrogen Fixation in Soybean Cultivars
Low-Density Co-Inoculation with Bradyrhizobium japonicum SAY3-7 and Streptomyces griseoflavus P4 Promotes Plant Growth and Nitrogen Fixation in Soybean Cultivars作者机构：Laboratory of Plant Nutrition Graduate School of Bioresource and Bioenvironmental Sciences Faculty of Agriculture Kyushu University Fukuoka Japan Department of Agronomy Yezin Agricultural University Yezin Myanmar
出 版 物：《American Journal of Plant Sciences》 (美国植物学期刊（英文）)
年 卷 期：2016年第7卷第12期
页 面：1652-1661页
主 题：Brayrhizobium japonicum SAY3-7 Streptomyces griseoflavus P4 Inoculation Densities Co-Inoculation Soybean Nitrogen Fixation
摘 要：Inoculation density has a marked effect on nodulation and N fixation in soybean (Glycine max L.). Therefore, we conducted this study to determine the optimal inoculation density of Bradyhizobium japonicum SAY3-7 (SAY3-7) and Streptomyces griseoflavus P4 (P4) for plant growth, nodulation, and N fixation, and to investigate the effect of co-inoculation on selected soybean cultivars, using the optimal inoculation density. Nitrogen fixation, in terms of an acetylene reduction activity value, was measured using a flame ionization gas chromatograph. In this study, low-density single inoculation with P4 (105 or 106 cells mL-1) was associated with the highest plant biomass, compared with normal- and high-density single inoculation with P4 (107 or 108 cells mL-1). Moreover, low-density single or co-inoculations with SAY3-7 and/or P4 produced the highest nodule biomass and highest nitrogenase activity, compared with single or dual inoculation at other inoculation densities. Therefore, we evaluated low-density co-inoculation with P4 and SAY3-7, at the rate of 105 cells mL-1, on selected soybean cultivars. Low-density co-inoculation increased the plant biomass, compared with un-inoculated plants. The effects of single and co-inoculation on nodulation did not differ significantly for any of the cultivars, except “Yezin-9 in the first experiment and “Shan Seine in the second experiment. Low-density inoculation with both bacteria increased N fixation by 15% - 75% for seven of the cultivars in the first experiment and by 15% - 39% for three of the cultivars in the second experiment, compared with single inoculation with SAY3-7. Based on the overall results, we concluded that low-density co-inoculation with P4 and SAY3-7 gave improved plant growth and N fixation.
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