Synthesis,Characterizations and in Vitro Assessment of the Cytotoxicity and Genotoxicity of Novel Silicon Nitride-Based Porous Ceramics

作     者：Miroslav Hnatko Zoltán Lences Peter Copan Lucia Birosová Patrik Matejov Sona Jantová 

作者机构：Department of CeramicInstitute of Inorganic ChemistrySlovak Academy of SciencesBratislavaSlovakia Institute of Inorganic ChemistryTechnology and MaterialsFaculty of Chemical and Food TechnologySlovak University of TechnologyBratislavaSlovakia Institute of BiochemistryNutrition and Health ProtectionFaculty of Chemical and Food TechnologySlovak University of TechnologyBratislavaSlovakia 

出 版 物：《Materials Sciences and Applications》 (材料科学与应用期刊（英文）)

年 卷 期：2013年第4卷第7期

页      面：407-418页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：This study was supported by the Technology Assistance Agency under the contract no.APVV-0500-10 the Slovak State Committee for Scientific Research VEGA,grant 1/0165/10 

主　　题：Silicon Nitride Sintering Porosity Mechanical Properties Cytotoxicity/Genotoxicity Human Cell Lines 

摘      要：Porous Si3N4-SiO2-based ceramics with different porosity were prepared via free sintering of Si3N4 on air with an addition of semolina (5, 10 and 20 wt%) as a pore-forming agent. The semolina content in the starting powder controlled the volume fraction of pores in the sintered body. Small pores (5 μm) formed a continuous network in the whole volume of the ceramic material, while the large pores (~100 μm), formed from the added semolina were mostly isolated in the ceramic matrix. Mercury porosimetry and strength measurements have shown that specific surface area, volume density and compressive strength decreased with the amount of semolina in the samples. Mechanical properties similar to bone were obtained for the sample with 20 wt% semolina pore forming agent (compressive strength 350 MPa, density 2.17 ***-3). The prepared Si3N4-SiO2-based ceramics were evaluated for cytotoxic and genotoxic potential on human fibroblast VH10 and B-HNF-1 cells. Biological tests have shown that both these human fibroblast cell lines were sensitive to the samples with lower porosity and cell growth inhibition was observed in the range 14.9% - 21.3%. The cytotoxicity of the sample with the highest porosity (~40%) was not significant (10%). The microscopic observations have shown that VH10 and B-HNF-1 cells growing around the silicon nitride ceramic discs were homogeneously distributed on the cultivation surface. No significant morphologic changes were found in treated cells, their morphology was very similar to that of the control cells. None of the tested Si3N4-based ceramic samples induced necrotic/apoptotic death of human fibroblasts. Sample S-20 had similar properties to bones and was characterized by very good biocompatibility, slight cytotoxicity and none genotoxicity. Therefore, Si3N4-SiO2-based ceramics prepared by free sintering on air are potential biomaterials for medical applications.