Organic Solvent-Free and Simple Method for Determining Cyromazine and Its Metabolite, Melamine, in Cow’s Milk
Organic Solvent-Free and Simple Method for Determining Cyromazine and Its Metabolite, Melamine, in Cow’s Milk作者机构：Graduate School of Human Life Science Osaka City University Osaka Japan
出 版 物：《Journal of Analytical Sciences, Methods and Instrumentation》 (分析科学方法和仪器期刊（英文）)
年 卷 期：2012年第2卷第2期
页 面：68-73页
主 题：Cyromazine Melamine HPLC-PDA Milk: Organic Solvent-free
摘 要：This paper described an organic solvent-free, rapid, simple, and space-saving method of sample preparation followed by HPLC coupled photo-diode array (PDA) detector for simultaneous quantification of cyromazine (CYR) and its decy-cropropylated metabolite, melamine (MEL), in milk. The HPLC-PDA was performed on an Inertsil? HILIC column with an isocratic aqueous mobile phase. Analytes were extracted from the sample using water, and purified by Mono-Spin?-C18, a centrifugal monolithic silica spin mini-columns, and quantified within 20 min. The method, performed under 100% aqueous conditions, obtained average recoveries for CYR and MEL in the range of 93.2% - 99.1% with relative standard deviations ≤ 2.8%. The quantitation limits were 8.5 ng/mL for CYR and 10 ng/mL for MEL, respectively. No organic solvents were used at any stage of the analysis.
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