Single Corn Kernel Aflatoxin B<sub>1</sub> Extraction and Analysis Method
Single Corn Kernel Aflatoxin B<sub>1</sub> Extraction and Analysis Method作者机构：Department of Biochemistry Molecular Biology Entomology and Plant Pathology Mississippi State University Oktibbeha County USA Mississippi State Chemical Laboratory Mississippi State University Oktibbeha County USA Corn Host Plant Resistance Research Unit United States Department of Agriculture-Agricultural Research Service Oktibbeha County USA
出 版 物：《Natural Resources》 (自然资源（英文）)
年 卷 期：2016年第7卷第7期
页 面：405-410页
学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学]
主 题：Aspergillus flavus Aflatoxin Maize HPLC MS/MS Extraction
摘 要：Aflatoxins are highly carcinogenic compounds produced by the fungus Aspergillus flavus. Aspergillus flavus is a phytopathogenic fungus that commonly infects crops such as cotton, peanuts, and maize. The goal of this research was to design an effective method for sample preparation and analysis of aflatoxin B1 from Aspergillus flavus infected maize kernels. The method involves liquid extraction with a methanol/water solution. The samples were analyzed by liquid chromatography coupled to an electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) operating in positive ion multiple reaction monitoring (MRM) mode. The spiked levels for the recovery experiment were 4 ppb and 20 ppb, respectively. Recoveries ranged between 72% and 113% (90.8% average), with Relative Standard Deviation below 15% (10.6% on average).
维普期刊数据库