High yield expression of proteins in <i>E. coli</i>for NMR studies
High yield expression of proteins in <i>E. coli</i>for NMR studies作者机构：NMR Research Centre Indian Institute of Science Bangalore India
出 版 物：《Advances in Bioscience and Biotechnology》 (生命科学与技术进展（英文）)
年 卷 期：2013年第4卷第6期
页 面：751-767页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：E. Coli Recombinant DNA Technology Structural Biology NMR Spectroscopy
摘 要：In recent years, high yield expression of proteins in E. coli has witnessed rapid progress with developments of new methodologies and technologies. An important advancement has been the development of novel recombinant cloning approaches and protocols to express heterologous proteins for Nuclear Magnetic Resonance (NMR) studies and for isotopic enrichment. Isotope labeling in NMR is necessary for rapid acquisition of high dimensional spectra for structural studies. In addition, higher yield of proteins using various solubility and affinity tags has made protein over-expression cost-effective. Taken together, these methods have opened new avenues for structural studies of proteins and their interactions. This article deals with the different techniques that are employed for over-expression of proteins in E. coli and different methods used for isotope labeling of proteins vis-à-vis NMR spectroscopy.
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