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Analyzing the Physico-Chemical Parameters of Detergents and Detergent Mixtures

Analyzing the Physico-Chemical Parameters of Detergents and Detergent Mixtures

作     者:Britta Tschapek Sander H. J. Smits Lutz Schmitt 

作者机构:Institute of Biochemistry Heinrich Heine University Duesseldorf Duesseldorf Germany 

出 版 物:《Advances in Chemical Engineering and Science》 (化学工程与科学期刊(英文))

年 卷 期:2015年第5卷第3期

页      面:328-337页

学科分类:07[理学] 0703[理学-化学] 

主  题:Detergent CMC Determination Fluorescence Mixed Micelles Aggregation Number 

摘      要:The most important physico-chemical characteristics describing detergent micelles are the CMC value and the aggregation number. These parameters depend critically on external conditions such as pH, ionic strength, buffer composition or temperature. However, the influence of these conditions on CMC or aggregation number is a priori not to predict, but the most important parameter. This holds especially for the aggregation number. Here, we present a simple, reliable and fast method for the analysis of detergent containing buffer systems. It enables the determination of CMC values and aggregation numbers of detergents as well as detergent mixtures in a 96well plate standard device relying on steady state fluorescence and fluorescence quenching. To prove the general applicability of our approach, we analyzed DDM as a prime example of a detergent and mixtures of DDM with six other detergents at different ratios to demonstrate the potential of our method.

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