Effect of Bak Foong Pills on the expression of β-amyloid in rat retina with optic nerve transection
Effect of Bak Foong Pills on the expression of β-amyloid in rat retina with optic nerve transection作者机构：Department of Ophthalmology the Second Xiangya Hospital Central South University Changsha 410011 Hunan Province China Department of Ophthalmology the Second People's Hospital of Yunnan Province Kunming 650021 Yunnan Province China
出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)
年 卷 期：2011年第4卷第1期
页 面：58-61页
核心收录：
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学]
主 题：Bak Foong Pills optic nerve damage β-amyloid protein Bcl-2/Bax Caspase-3 mRNA
摘 要：AIM:To investigate the effect of Bak Foong Pills(BFP) on the expression of β-amyloid(Aβ) in rats retina with optic nerve transaction,and its roles and possible mechanisms in protecting optic nerve damage.· METHODS:Seventy-two healthy,Sprague-Dawley,adult rats were randomly assigned to three groups:negative control group(control group),optic nerve transection group(model group) and BFP treatment group(BFP group,100μg/mL) followed by establishing optic nerve transection *** expression of Aβ was measured at 48 hours by ***,the expressions of Bcl-2,Bax and Caspase-3 mRNA were evaluated at 48 hours by reverse transcriptase polymerase chain reaction(RT-PCR).RESULTS:There were significant differences among the control,model and BFP groups in the expression of Aβ(all P 0.01).Aβ expression was significantly higher in the model and BFP groups than that in the control group(P 0.01),with a more significant reduction in the BFP group than that in the model group(P 0.01).Moreover,there were also significant differences among the three groups in the expressions of Bcl-2/Bax(Bcl-2:anti-apoptotic;Bax:proapoptotic) and Caspase-3 mRNA(proapoptotic)(all P0.01).Bcl-2/Bax ratio was significantly lower and Caspase-3 mRNA expression was significantly higher in the model and BFP groups than those in the control group(P 0.01),with a significant growing of Bcl-2/Bax and reduction of Caspase-3 in the BFP group than those in the model group(P0.01).· CONCLUSION:BFP can down-regulate Aβ expression in retina and may inhibit apoptosis and protect optic nerve by enhancing Bcl-2/Bax ratio and inhibiting Caspase-3 pathway.
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