LOH analysis of genes around D4S2964 identifies ARD1B as a prognostic predictor of hepatocellular carcinoma

作     者：Huang, Guo-Liang Li, Bin-Kui Zhang, Mei-Yin Zhang, Hui-Zhong Wei, Rong-Rong Yuan, Yun-Fei Shi, Ming Chen, Xiao-Qian Huang, Long Li, An-Hua Huang, Bi-Jun Li, Hong-Hua Wang, Hui-Yun 

作者机构：Sun Yat Sen Univ Ctr Canc State Key Lab Oncol S China Guangzhou 510060 Guangdong Peoples R China Sun Yat Sen Univ Ctr Canc Dept Hepatobiliary Oncol Guangzhou 510060 Guangdong Peoples R China Sun Yat Sen Univ Ctr Canc Dept Pathology Guangzhou 510060 Guangdong Peoples R China Sun Yat Sen Univ Ctr Canc Dept Ultrasound & Electrocardiogram Guangzhou 510060 Guangdong Peoples R China UMDNJ Robert Wood Johnson Med Sch Canc Inst New Jersey Dept Microbiol Mol Genet & Immunol Piscataway NJ 08854 USA 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2010年第16卷第16期

页      面：2046-2054页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Supported by National Natural Science Foundation of China,No. 30772491 to Wang HY partially supported by Research Fund of State Key Laboratory of Oncology in South China to Wang HY 

主　　题：ARD1 homolog B Hepatocellular carcinoma Loss of heterozygosity Septin 11 Single nucleotide polymorphism 

摘      要：AIM:To investigate genes around the locus D4S2964 affected by loss of heterozygosity(LOH) and their clinical ***:Four hundred and forty single nucleotide polymorphisms(SNPs) located at 49 genes around D4S2964 were selected from the National Center for Biotechnology Information website for the SNPs microarray *** of SNPs markers in 112 cases of hepatocellular carcinoma(HCC) tissues and paired adjacent liver tissues were investigated by the SNPs *** correlation between allelic losses with clinicopathological features and overall survival was ***:A f ine map of LOH of SNPs in genes around D4S2964 was *** average frequency of LOH in genes was 0.39.A correlation between cirrhosis and the FAL index(fractional allelic loss) was found(P = 0.0202).Larger tumor size was found to be signif icantly associated with LOH in genes ADP-ribosyltransferase 3(ART3),nucleoporin 54 kDa(NUP54),scavenger receptor class B,member 2(SCARB2) and coiled-coil domain containing 158(CCDC158)(P = 0.043,P = 0.019,P = 0.001,P = 0.037,respectively).Kaplan-Meier analysis showed that patients with LOH in ARD1 homolog B(ARD1B) and septin 11(SEPT11) had a significantly lower survival rate than those with retention(P = 0.021 and P = 0.004,respectively).A Cox regression model suggested that LOH in ARD1B and SEPT11,respectively,were predictors of the overall survival in HCC(P = 0.006 and P = 0.026,respectively).CONCLUSION:LOH in genes around D4S2964 may play an important role in HCC development and *** in ARD1B and SEPT11 could serve as novel prognostic predictors in HCC patients.