A Study on Uptake and Localization of Merocyanine 540 (MC540) in Murine Myeloid Leukemia Cells by Confocal Laser Scanning Microscopy (CLSM)

作     者：J. Y. Chen-1 N. H. Cheung-2 S. C. Chen-2, M. C. Fung-3 W. N. Leung-4 N. K. Mak- 4+ ( -1Department of Physics, Fudan University, Shanghai 200433, China -2Department of Physics, Hong Kong Baptist University, Hong Kong, China -3Department of Bi 

作者机构：复旦大学 上海 香港浸会大学 香港 香港大学 香港 

出 版 物：《Chinese Journal of Lasers》 (中国激光(英文版))

年 卷 期：2000年第9卷第3期

页      面：35-41页

核心收录：

学科分类：0808[工学-电气工程] 080901[工学-物理电子学] 0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学] 080401[工学-精密仪器及机械] 0804[工学-仪器科学与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 0803[工学-光学工程] 0702[理学-物理学] 

基　　金：SupportedbytheFacultyResearchGrantofHKBU  theCroucherFoundation  andtheNationalNaturalScienceFoudationofChina 

主　　题：merocyanine 540 Confocal Laser Scanning Microscopy Myeloid Leukemia cell 

摘      要：The intracellular localization of merocyanine 540 (MC540), a photosensitizer commonly used in the photo-inactivation of leukemia cells, was studied using confocal laser scanning microscopy. It was found for the first time that MC540 not only localized in the plasma membrane but also in the cytoplasm and the nuclear membrane of the murine myeloid leukemia M1 and WEHI 3B (JCS) cells. Exposure of MC540 treated leukemia cells to light under conditions that could cause photobleaching did not cause the redistribution of cell-bound MC540. Rapid localization of MC540 in the cytoplasm was observed 5 minutes after exposure of leukemia cell to MC540, indicating that MC540 could promptly be internalized by these two leukemia cell lines. In contrast, localization of MC540 was limited only to the plasma membrane of erythrocytes. These results suggest that the binding pattern of MC540 is cell type dependent and may be related to the efficacy of photosenitization in photodynamic therapy.