Application of glutathione to antagonize H2O2-induced oxidative stress in rat tracheal epithelial cells

作     者：Jian Zou Jinbo Yu Yuqing Zhu Jiali Zhu Jing Du Xu Yang 

作者机构：School of Life Sciences Central China Normal University Wuhan 430079 China 

出 版 物：《Frontiers in Biology》 (生物学前沿（英文版）)

年 卷 期：2016年第11卷第1期

页      面：59-63页

核心收录：

学科分类：0710[理学-生物学] 083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 08[工学] 09[农学] 0903[农学-农业资源与环境] 071002[理学-动物学] 0713[理学-生态学] 

主　　题：H2O2 glutathione oxidative stress TSLP 

摘      要：With increasing industrialization, numerous air pollutants are generated. This research aimed to investigate the effects of inhalation of oxidative pollutants. H2O2 was used to simulate oxidative air pollutants, and glutathione, a reducing agent that is widely distributed in organisms, was used as an antagonist, to protect cells from oxidative stress. H2O2 was diluted using two gradients （0.05 mM, 0.20 mM, 0.80 mM, 3.20 mM and 0.05 mM, 0.10 mM, 0.15 mM, 0.20 mM） and GSH was dissolved at 20 μM. MTT, MDA, ROS, GSH, and TSLP were used as biomarkers to evaluate oxidative stress and possible resulting molecular events. A dose-response relationship was observed between H2O2 concentrations and the above-mentioned biomarkers. Glutathione significantly reduced levels of oxidative stress.