Immune Responses to the Attenuated Mycoplasma hyopneumoniae 168 Strain Vaccine by Intrapulmonic Immunization in Piglets

作     者：FENG Zhi-xin, SHAO Guo-qing, LIU Mao-jun, WU Xu-su, ZHOU Yong-qi and GAN Yuan Key Laboratory of Animal Diseases Diagnostic and Immunology, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences/National Center for Engineering Research of Veterinary Bio-Products, Ministry of Agriculture, Nanjing 210014, P.R.China 

作者机构：[a] Key Laboratory of Animal Diseases Diagnostic and Immunology Institute of Veterinary Medicine Jiangsu Academy of Agricultural Sciences/National Center for Engineering Research of Veterinary Bio-Products Ministry of Agriculture Nanjing 210014 P.R.China 

出 版 物：《Agricultural Sciences in China》 (中国农业科学（英文版）)

年 卷 期：2010年第9卷第3期

页      面：423-431页

核心收录：

学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：supported by the Natural Science Foundation of Jiangsu Province, China (BK2007711) the China Postdoctoral Fundation (20070421022) the Three Agricultural Projects Fundation of Jiangsu Province, China (SX2007082) 

主　　题：Mycoplasma hyopneumoniae attenuated vaccine cellular immunity mucosal immunity 

摘      要：To investigate the immune responses to the attenuated Mycoplasma hyopneumoniae 168 strain vaccine, 8-15 d old piglets were immunized with M. hyopneurnoniae 168 strain vaccine by intrapulmonic route. And the specific IgG antibody in serum, lymphoproliferation, IFNT, and specific secretory IgA （SIgA） antibody in bronchoalveolar lavage fluid were detected on 30 and 60 d post-immunization （DPI）, respectively. On 60 DPI, all the pigs except for those in health control group were challenged with a field M. hyopneumoniae strain JS. Necropsy was performed on 30 d post-challenge （DPC）. The results showed that IFN7 and specific SIgA were stimulated on surface of respiratory tract after immunization. And peripheral blood mononuclear cells could also be proliferated about 1.81 and 2.12 fold on 30 and 60 DPI when stimulated by M. hyopneumoniae protein in vitro. However, no serum IgG antibody against M. hyopneumoniae was detected during the whole immune phage. After challenge, vaccinated pigs were observed with only very slight histological lesion in individual lobes. None of vaccinated pigs showed any clinical signs. While the unvaccinated pigs from challenge control group showed varying degrees of clinical sign and severe macroscopical lesion of mycoplasmal pneumonia of swine （MPS）. The result suggested that the attenuated M. hyopneumoniae 168 strain vaccine inoculated by intrapulmonic route could activate the systemic cellular immunity, the local mucosal immunity and IFNγ secretion in respiratory tract to against M. hyopneumoniae infection in piglets.