Association between vascular endothelial growth factor gene promoter polymorphisms and sporadic amyotrophic lateral sclerosis in a Han Chinese population Case-control study

作     者：Na Liu Nan Zhang Jun Zhang Liping Wang Xiaoyan Zhang Wei Sui Huagang Zhang Lingsong Li Dafang Chen Dongsheng Fan 

作者机构：Department of Neurology Peking University Third Hospital Beijing 100191 China Peking University Stem Cell Research Center Beijing 100191 China Department of Epidemiology and Statistics School of Public Health Peking University Beijing 100191 China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2009年第4卷第12期

页      面：1110-1115页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

主　　题：amyotrophic lateral sclerosis genetic polymorphisms vascular endothelial growth factor 

摘      要：BACKGROUND： Studies have shown that vascular endothelial growth factor （VEGF） gene polymorphisms highly correlate with sporadic amyotrophic lateral sclerosis （ALS）, although this remains controversial. To date, the relationship between VEGF gene polymorphism and sporadic ALS in a Han Chinese population remains unclear. OBJECTIVE： To explore the relationship between sporadic ALS and VEGF gene promoter 7 locus polymorphisms in a Hart Chinese population, and to investigate whether this relationship exhibits gender differences. DESIGN, TIME AND SETTING： A case-control study regarding genetic association was performed at the Central Laboratory of Peking University Third Hospital from 2002 to 2006. PARTICIPANTS： A total of 93 patients, who were diagnosed with definite or probable ALS according to E1 Escorial-revised diagnostic criteria, were selected from the Outpatient Department of Neurology, Peking University Third Hospital from 2002 to 2006. All patients were from Hart populations, with no family history of ALS. In addition, 103 gender- and age-matched healthy volunteers were selected as controls. METHODS： Peripheral venous blood was collected, and whole blood genomic DNA was extracted. VEGF gene promoter 7 locus polymorphisms were analyzed by PCR using the fluorescent Taqman system. The relationship between VEGF gene promoter single nucleotide polymorphisms and ALS was analyzed using Logistic regression model analysis and was stratified according to gender. MAIN OUTCOME MEASURES： 7 VEGF gene promoter polymorphisms genotype distribution and allele frequencies. RESULTS： There were no significant differences in VEGF gene promoter 7 locus genotype distribution and allele frequency between case and control groups （P 〉 0.05）. Stratified analysis based on gender demonstrated that female Han subjects carrying the VEGF genotype -1154AA, -2549TT, -634CC genotype were more susceptible to ALS than those carrying -1154GG, -2549CC, -634GG （VEGF-1154AA： OR= 8.9, 95%CI= 1 .0-77.3, P