Evaluation of maize inbred lines and topcross progeny for resistance to pre-harvest aflatoxin contamination

作     者：Jake C.Fountain Hamed K.Abbas Brian T.Scully Hong Li Robert D.Lee Robert C.Kemerait Baozhu Guo 

作者机构：USDA-ARS Crop Protection and Management Research Unit Department of Plant Pathology University of Georgia USDA-ARS Biological Control of Pests Research Unit USDA-ARS National Horticultural Research Laboratory Shanxi Academy of Agricultural Sciences Millet Research Institute Department of Crop and Soil Sciences University of Georgia 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2019年第7卷第1期

页      面：118-125页

核心收录：

学科分类：09[农学] 

基　　金：partially supported by the U.S.Department of Agriculture, Agricultural Research Service (USDA-ARS) the Georgia Agricultural Commodity Commission for Corn AMCOE (Aflatoxin Mitigation Center of Excellence, Chesterfield, MO, USA) 

主　　题：Aspergillus flavus Pre-harvest Aflatoxin Maize Topcross 

摘      要：Pre-harvest aflatoxin contamination occurs in maize following kernel colonization by Aspergillus flavus. Aflatoxin contamination resistance is a highly desired trait in maize breeding *** identification of novel sources of resistance to pre-harvest aflatoxin contamination is a major focus in germplasm screening efforts. Here, we performed a field evaluation of 64 inbred lines over two years for pre-harvest aflatoxin contamination. Topcrosses were also performed with two testers, B73 and Mo17, to generate 128 F1 hybrids which were also evaluated over two years. Hybrid performance was used to calculate both general combining ability(GCA) of the inbreds, and observed heterosis for aflatoxin resistance. Over both years of the study, aflatoxin concentrations ranged from 80 ± 47 to 17,617 ± 8816 μg kg^(-1) for inbreds, and from 58 ± 39 to 2771 ± 780 μg kg^(-1) for hybrids with significant variation between years and lines. The inbred lines CML52, CML69, CML247, GT-603, GEMS-0005, Hi63, Hp301, and M37 W showed 1000 μg kg^(-1) of aflatoxin accumulation in both years of the study and less than the resistant check, Mp313 E, in at least one season. Among these, CML52, GT-603, and Hi63 also showed significant GCA with the testers in hybrid progeny. CML52, GT-603, and M37 W also showed heterotic effects of-13.64%,-12.47%, and-24.50%, respectively, with B73 resulting in reduced aflatoxin contamination. GT-603 also showed a similar heterotic effect for aflatoxin contamination,-13.11%, with Mo17 indicating that this line may serve as a versatile source of aflatoxin contamination resistance in breeding programs.