Targeted migration and differentiation of engrafted neural precursor cells in amyloid β-treated hippocampus in rats

作     者：唐军 徐海伟 范晓棠 李志方 李达兵 杨丽 周光纪 Jun TANG;Hai-Wei XU;Xiao-Tang FAN;Zhi-Fang LI;Da-Bing LI;Li YANG;Guang-Ji ZHOU

作者机构：第三军医大学基础医学部生理学教研室 第三军医大学基础医学部生理学敦研室 第三军医大学基础医学部神经生物学教研室重庆400038 

出 版 物：《Neuroscience Bulletin》 (神经科学通报（英文版）)

年 卷 期：2007年第23卷第5期

页      面：263-270页

核心收录：

学科分类：1002[医学-临床医学] 100203[医学-老年医学] 100204[医学-神经病学] 10[医学] 

基　　金：This work was supported by the National Natural Science Foundation of China (No. 30571770) 

主　　题：embryonic stem cells amyloid β peptide cell transplantation differentiation migration rat 

摘      要：Objective To observe the migration and differentiation of the neural precursor cells （NPCs） that derived from murine embryonic stem cells （ESCs） when they were transplanted into amyloid β （Aβ）-treated rat hippocampus. Methods MESPU35, a murine ESC cell line that express the enhanced green fluorescent protein （EGFP）, was induced differentiation into nestin-positive NPCs by modified serum-free methods. The Aβ plaques and the differentiation of the grafted cells were observed by immunofluorescent staining. Results Comparing 16 weeks with 4 weeks post-transplantation, the migration distance increased about 5 times; the rate of migratory NPCs differentiating into glial fibrillary acidic protein （GFAP）-positive cells kept rising from （30.41 ± 1.45）% to （49.25± 1.23）%, and the rate of NPCs differentiating into neurofilament 200 （NF200） positive cells increased from （16.68±0.95）% to （27.94± 1.21）%. Meanwhile, the GFAP-positive cells targeting to the ipsilateral side of Aβ plaques increased from 60.2% to 81.3 %, while the NF200-positive cells increased from 61.3% to 84.1%. The migration distance had significant positive linear correlations to the neuronal differentiation rate （r = 0.991） and to the astrocytic differentiation rate （r = 0.953）. Conclusion Engrafted NPCs migrate targetedly to the Aβ injection site and differentiate into neurons and astrocytes.