Rapid Diagnosis with FISH for Chromosomal Abnormality of Fetal Pyelectasia

作     者：HUANG Fenghua ZHENG Xingming ZHANG Yuanzheng XIAOLiping LIN Li 

作者机构：Department of Obstetrics and Gynecology ZhongnanHospital Wuhan University Wuhan 430071 Hubei China Department of Urology Zhongnan Hospital WuhanUniversity Wuhan 430071 Hubei China 

出 版 物：《Wuhan University Journal of Natural Sciences》 (武汉大学学报（自然科学英文版）)

年 卷 期：2008年第13卷第2期

页      面：252-256页

学科分类：1002[医学-临床医学] 100211[医学-妇产科学] 10[医学] 

基　　金：the Key Program of Science and Technology of Wuhan(200760423158) 

主　　题：fluorescence in situ hybridization fetus pyelectasia chromosomal abnormality amniotic fluid cell 

摘      要：Fluorescence in situ Hybridization （FISH） was used to investigate whether the chromosome of the fetus prenatally diagnosed as pyelectasis was normal or not. Amniotic fluid was taken from the pregnant woman whose fetus was detected with pyelectasia by prenatal examination. The chromosome of the amniotic fluid cell without culture was examined with FISH. The result shows that compared with the traditional amniotic fluid cell culture, FISH has the advantages of more rapid, higher sensitivity and specificity, and was 10-12 days earlier to complete the diagnosing than the traditional method. The fetuses detected chromosomal abnormality in each groups were induced during the middle and late trimester, while those fetuses with normal chromosome continued pregnancy, the rate of spontaneous disappearance of pyelectasia decreased as the severity of pyelectasia increased. FISH can satisfy the urgent need in the clinical prenatal diagnosis due to its rapidity to determine whether fetus with pyelectasia was accompanied with chromosomal.