Identification and Purity Test of Super Hybrid Rice with SSR Molecular Markers
Identification and Purity Test of Super Hybrid Rice with SSR Molecular Markers作者机构：SchoolofLifeScienceandTechnologyCentralSouthUniversiryChangsha410083China／／ChinaNationalHybridRiceResearchandDevelopmentalCenterChangsha410125China ChinaNationalHybridRiceResearchandDevelopmentalCenterChangsha410125China
出 版 物：《Rice science》 (水稻科学（英文版）)
年 卷 期：2005年第12卷第1期
页 面：7-12页
主 题：SSR molecular marker super hybrid rice identification purity
摘 要：Five super hybrid rice combinations, i.e. HYS-1/R105, Pei ai 64S/E32, Liangyoupeijiu (Pei ai 64S/9311), 88S/0293, and J23A/Q611, and their parental lines were tested by means of SSR analysis. A total of 144 SSR primer pairs distributed on 12 rice chromosomes were used, out of which 47 detected polymorphism among the tested rice lines. Among all these primers, RM337 and RM154 produced polymorphic patterns in four or more of the tested experimental materials respectively, and they could distinguish among most rice genotypes tested. Twenty-four primer pairs, two on each rice chromosome, were selected to make a reference SSR marker-based fingerprinting for the rice lines. For most of the primer pairs, F1 hybrids mainly showed complementary pattern of both parents, which could be very useful to distinguish the F1 from its parental lines. In addition, 5 primer pairs were selected as special primer pairs for five hybrid rice combinations respectively. By combining the rapid, simple method on DNA extraction, it is suggested that SSR technique has wide prospective in variety authentication and purity identification.
维普期刊数据库
同方期刊数据库