利用ISSR DNA标记鉴定主要银杏栽培品种
Identification on the Main Cultivated Varieties of Ginkgo biloba Using ISSR DNA Marker作者机构：南京林业大学国家林业局南方林木种子检验中心南京210037 江苏省邳州市林业局邳州221400
出 版 物：《林业科学》 (Scientia Silvae Sinicae)
年 卷 期：2005年第41卷第1期
页 面：202-204页
核心收录：
学科分类：07[理学] 09[农学] 0901[农学-作物学] 0713[理学-生态学]
主 题：ISSR 栽培品种 银杏 利用 RAPD 鉴定 DNA
摘 要：Ginkgo biloba is one of most famous officinal plant in China. In the past, many varieties were selected and planted, but there is not still identification system for these varieties. This paper studied DNA fingerprints of the main G. biloba varieties cultivated all over the country using ISSR DNA marker. The results indicated ISSR marker was effective highly for identifying 13 varieties. Only using primer ISSR46 and ISSR44, 13 varieties were able to be identified based on 11 polymorphic loci (ISSR46-550,ISSR46-670,ISSR46-740,ISSR46-1000,ISSR46-1159,ISSR46-1359,ISSR46-1600,ISSR44-520,ISSR44-580,ISSR44-660,ISSR44-1750). In addition, we found ISSR marker was stable and repeatable highly compared with RAPD marker.
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