Voltage-gated K^+channels promote BT-474 breast cancer cell migration

作     者：Louis WC Chow Ka-Shun Cheng Kar-Lok Wong Yuk-Man Leung 

作者机构：State Key Laboratory of Quality Research in Chinese MedicinesMacao University of Science and TechnologyTaipaMacao 999078China UNIMED Medical InstituteHong Kong 999077China Organisation for Oncology and Translational ResearchHong Kong 999077China Department of AnesthesiologyChina Medical University HospitalTaiehung 40447TaiwanChina Department of Anesthesiologythe Qingdao University Yuhuangding HospitalYantai 264000China Department of PhysiologyChina Medical UniversityTaichung 40402TaiwanChina 

出 版 物：《Chinese Journal of Cancer Research》 (中国癌症研究（英文版）)

年 卷 期：2018年第30卷第6期

页      面：613-622页

核心收录：

学科分类：10[医学] 

基　　金：funded by the Macao Science and Technology Development Fund(FUNDO PARA O DESENVOLVIMENTO DAS CIêNCIAS E DA TECNOLOGIA)and the reference number was 002/2015/A1 

主　　题：Breast cancer Kv channels migration biomarkers 

摘      要：Objective: A variety of ion channels have been implicated in breast cancer proliferation and *** K+(Kv) channels not only cause repolarization in excitable cells,but are also involved in multiple cellular functions in non-excitable *** this study we investigated the role of Kv channels in migration of BT474 breast cancer ***: Transwell technique was used to separate migratory cells from non-migratory ones and these two groups of cells were subject to electrophysiological examinations and microfluorimetric measurements for cytosolic *** migration was examined in the absence or presence of Kv channel ***: When compared with non-migratory cells,migratory cells had much higher Kv current densities,but rather unexpectedly,more depolarized membrane potential and reduced *** transcriptasepolymerase chain reaction(RT-PCR) analysis revealed the presence of Kv1.1,Kv1.3,Kv1.5,Kv2.1,Kv3.3,Kv3.4 and Kv4.3 *** migration was markedly inhibited by tetraethylammonium(TEA),a delayed rectifier Kv channel blocker,but not by 4-aminopyridine,an A-type Kv channel ***: Taken together,our results show that increased Kv channel expression played a role in BT474 cell migration,and Kv channels could be considered as biomarkers or potential therapeutic targets for breast cancer *** mechanism(s) by which Kv channels enhanced migration appeared unrelated to membrane hyperpolarization and Cainflux.