Novel circular RNA expression profile of uveal melanoma revealed by microarray

作     者：Xuan Yang Yang Li Yueming Liu Xiaolin Xu Yingzhi Wang Yanni Yan Wenjia Zhou Jingyan Yang Wenbin Wei 

作者机构：Beijing Tongren Eye CenterBeijing Key Laboratory of Intraocular Tumor Diagnosis and TreatmentBeijing Ophthalmology & Visual Sciences Key LabBeijing Tongren HospitalCapital Medical University Beijing Institute of OphthalmologyBeijing Key Laboratory of Intraocular Tumor Diagnosis and TreatmentBeijing Tongren Eye CenterBeijing Tongren HospitalCapital Medical UniversityBeijing Ophthalmology & Visual Sciences Key Laboratory 

出 版 物：《Chinese Journal of Cancer Research》 (中国癌症研究（英文版）)

年 卷 期：2018年第30卷第6期

页      面：656-668页

核心收录：

学科分类：10[医学] 

基　　金：supported by Beijing Municipal Administration of Hospitals’ Ascent Plan(No.DFL20150201) the National Natural Science Foundation of China(No.81570891) Beijing Natural Science Foundation(No.7151003) Advanced Health Care Professionals Development Project of Beijing Municipal Health Bureau(No.2014-2-003) The Capital Health Research and Development of Special(No.2016-1-2051) 

主　　题：Uveal melanoma circular RNA microarray noncoding RNA 

摘      要：Objective: The present study aimed to investigate circular RNA(circRNA) expression in uveal melanoma(UM).Methods: First,we used microarray to compare the expression profiles of circRNA in five UM samples and five normal uvea ***,bioinformatics analyses,including gene ontology(GO) analysis and pathway analysis,were applied to study these differentially expressed circRNAs to predict pathogenic pathways that may be *** real-time polymerase chain reaction(qRT-PCR) in 20 UM samples and 20 normal uvea samples was used to confirm the circRNA expression profiles obtained from the microarray ***,we analyzed the interaction between validated circRNAs and their potential cancer-associated miRNA ***: In total,50,579 circRNAs [fold change(FC) ≥2.0; P0.05],including 20,654 up-regulated and 29,925 down-regulated circRNAs,were identified as differentially expressed between UM tissues and normal uvea *** used qRT-PCR to verify seven dysregulated circRNAs indicated by the microarray data,including hsacirc0119873,hsacirc0128533,hsacirc0047924,hsacirc0103232,hsa-circRNA10628-6,hsacirc0032148 and hsacirc0133460,which may be promising candidates to study future molecular ***: This study explored,for the first time,the abnormal expression of circRNAs in UM and described the expression profile of circRNAs,providing a new potential target for the mechanism of UM and future treatment of UM.