Stable-isotope probing of bacterial community for dissolved inorganic carbon utilization in Microcystis aeruginosa-dominated eutrophic water

作     者：Weiguo Zhang Jiangye Li Chengcheng Wang Xue Zhou Yan Gao Zhongjun Jia 

作者机构：Institute of Agricultural Resources and Environment Jiangsu Academy of Agricultural Sciences Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base China Ministry of Agriculture Key Laboratory at Yangtze River Plain for Agricultural Environment College of Resources and Environmental Sciences Nanjing Agricultural University College of Water Conservancy and Hydropower Engineering Hohai University Institute of Soil Science Chinese Academy of Sciences 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2019年第31卷第5期

页      面：264-272页

核心收录：

学科分类：08[工学] 0815[工学-水利工程] 

基　　金：supported by the National Key Research and Development Program of China(No.2017YFD0800101) the State Natural Science Foundation of China(Nos.31600419,41571458,41471415) 

主　　题：Dissolved inorganic carbon Stable isotope probing Eutrophication Microcystis aeruginosa 

摘      要：Dissolved inorganic carbon(DIC) is an important source of carbon in aquatic ecosystems,especially under conditions of increased frequency of cyanobacterial bloom. However, the importance of bacteria in direct or indirect utilization of DIC has been widely overlooked in eutrophic freshwater. To identify the functional bacteria that can actively utilize DIC in eutrophic freshwater during cyanobacterial bloom, stable-isotope probing(SIP) experiments were conducted on eutrophic river water with or without inoculation with cyanobacteria(Microcystis aeruginosa). Our 16 S rRNA sequencing results revealed the significance of Betaproteobacteria, with similar relative abundance as Alphaproteobacteria, in the active assimilation of H^(13)CO^(3-) into their DNA directly or indirectly, which include autotrophic genera Betaproteobacterial ammonia-oxidizing bacteria. Other bacterial groups containing autotrophic members, e.g. Planctomycetes and Nitrospira, also presented higher abundance among free-living bacteria in water without cyanobacteria. Microcystis aggregates showed a preference for some specific bacterial members that may utilize H^(13)CO^(3-) metabolized by Microcystis as organic matter, e.g. Bacteroidetes(Cytophagales, Sphingobacteriales), and microcystindegrading bacteria Betaproteobacteria(Paucibacter/Burkholderiaceae). This study provides some valuable information regarding the functional bacteria that can actively utilize DIC in eutrophic freshwater.