Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber

作     者：Sebastian A.Vasquez-Lopez Raphaël Turcotte Vadim Koren Martin Plöschner Zahid Padamsey Martin J.Booth TomášČižmár Nigel J.Emptage 

作者机构：Department of PharmacologyUniversity of OxfordMansfield RoadOxford OX13QTUK Department of Engineering ScienceUniversity of OxfordParks RoadOxford OX13PJUK School of EngineeringPhysics and MathematicsCollege of ArtScience&EngineeringUniversity of DundeeNethergateDundee DD14HN ScotlandUK. Institute of Scientific Instruments of the CASKrálovopolská14761264 BrnoCzech Republic 

出 版 物：《Light(Science & Applications)》 (光（科学与应用)（英文版）)

年 卷 期：2018年第7卷第1期

页      面：1-6页

核心收录：

学科分类：080901[工学-物理电子学] 0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学] 080401[工学-精密仪器及机械] 0804[工学-仪器科学与技术] 0803[工学-光学工程] 

基　　金：support from the University of Dundee and Scottish Universities Physics Alliance(PaLS initiative) support from the European Regional Development Fund,Project No.CZ.02.1.01/0.0/0.0/15003/0000476 support from the John Fell Fund,the BBSRC(TDRF) the MRC(UK) 

主　　题：multimode fiber resolution 

摘      要：Achieving intravital optical imaging with diffraction-limited spatial resolution of deep-brain structures represents an important step toward the goal of understanding the mammalian central nervous system1–*** in wavefrontshaping methods and computational power have recently allowed for a novel approach to high-resolution imaging,utilizing deterministic light propagation through optically complex media and,of particular importance for this work,multimode optical fibers(MMFs)5–*** report a compact and highly optimized approach for minimally invasive in vivo brain imaging *** volume of tissue lesion was reduced by more than 100-fold,while preserving diffraction-limited imaging performance utilizing wavefront control of light propagation through a single 50-μm-core ***,we demonstrated high-resolution fluorescence imaging of subcellular neuronal structures,dendrites and synaptic specializations,in deep-brain regions of living mice,as well as monitored stimulus-driven functional Ca2+*** results represent a major breakthrough in the compromise between high-resolution imaging and tissue damage,heralding new possibilities for deep-brain imaging in vivo.