Bcl-2 enhances the formation of newborn striatal long-projection neurons in adult rat brain after a transient ischemic stroke

作     者：Jian-Jun Guo Fang Liu Xiao Sun Jun-Jie Huang Ming Xu Feng-Yan Sun 

作者机构：Department of Neurobiology and Institute of Stem Cell ResearchInstitutes for Biomedical ScienceShanghai Medical CollegeFudan University State Key Laboratory of Medical Neurobiology of Fudan University 

出 版 物：《Neuroscience Bulletin》 (神经科学通报（英文版）)

年 卷 期：2012年第28卷第6期

页      面：669-679页

核心收录：

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

基　　金：supported by grants from the National Basic Research Development Program of China (2006CB504100 and 2006CB943702) the National Natural Science Foundation of China(81030020 and J0730860) 

主　　题：cerebral ischemia Bcl-2 striatonigral projection neuron neurogenesis brain repair 

摘      要：Objective It has been reported that B-cell lymphoma 2 （Bcl-2） enhances neurogenesis as well as supporting axonal growth after injury. In the present study, we investigated whether Bcl-2 overexpression plays a role in the formation of newborn striatonigral projection neurons in the adult rat brain after transient middle cerebral artery occlusion （MCAO）. Methods We infused human Bcl-2-expressing plasmid （pBcl-2） into the lateral ventricle immediately after 30 min of MCAO, injected 5＇-bromodeoxyuridine （BrdU） intraperitoneally to label proliferative cells, and microinjected fluorogold （FG） into the substantia nigra at 11 weeks of reperfusion followed by multiple immunostaining of striatonigral projection neurons at 12 weeks. Results We found that pBcl-2 treatment significantly increased the number of newborn neurons （BrdU＋-NeuN＋） in the striatum ipsilateral to the MCAO. We further detected newborn striatonigral projection neurons （BrdU＋-FG＋-NeuN＋） in the ipsilateral striatum at 12 weeks. More interestingly, the number of newborn striatonigral projection neurons （BrdU＋-FG＋） was significantly increased by pBcl-2 treatment compared to that by pEGFP, a control plasmid. Conclusion Taken together, we found that Bcl-2 overexpression in the brain enhanced the generation of newborn striatonigral projection neurons. This provides a potential strategy for promoting the reestablishment of neural networks and brain repair after ischemic injury.