Determination of Sex Hormones in Antler Velvet by High Performance Liquid Chromatography Tandem Mass Spectrometry

作     者：LU Chun-mei WANG Ming-tai MU Jun BAI Yu-ping DU Jian-shi ZHANG Han-qi WANG Jian-wei 

作者机构：College of ChemistryJilin UniversityChangchun 130012P.R.China China-Japan Union Hospital of Jilin UniversityChangchun 130021P.R.China Jilin Entry-exit Inspection and Quarantine BureauChangehun 130061R R.China China FAW Group Corporation R&D CenterChangchun 130011P.R.China Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022P.R.China 

出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)

年 卷 期：2012年第28卷第2期

页      面：191-194页

核心收录：

学科分类：07[理学] 08[工学] 082204[工学-皮革化学与工程] 070302[理学-分析化学] 0703[理学-化学] 0822[工学-轻工技术与工程] 

基　　金：Supported by the Projects of the General Administration of Quality Supervision Inspection and Quarantine of China(No.2007IK157) 

主　　题：Antler velvet Liquid chromatography tandem mass spectrometry Sex hormone Solid-phase extraction 

摘      要：Eighteen sex hormones in antler velvet were determined by high performance liquid chromatography tandem mass spectrometry.The solid phase extraction was applied to eliminating the matrix effect.The experimental conditions were examined and optimized.Under the optimal conditions,the proposed method provides the good linearities and determination limits（0.2―1.0 μg/kg） of the analytes investigated.The recoveries ranging from 72.3% to 149.5% were obtained for the target analytes at two concentration levels.This method was applied to the determination of eighteen sex hormones in different kinds of antler velvet samples and the obtained results are satisfactory.The results indicate that the proposed method is suitable for the determination of sex hormones in antler velvet samples.