Identification and functional characterization of an odorant receptor in pea aphid,Acyrthosiphon pisum

作     者：Rui-Bin Zhang Yang Liu Shan-Chun Yan Gui-Rong Wang 

作者机构：The College of ForestryNortheast Forestry UniversityHarbinChina State Key Laboratory for Biology of Plant Diseases and Insect PestsInstitute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingChina 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2019年第26卷第1期

页      面：58-67页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 09[农学] 0904[农学-植物保护] 0901[农学-作物学] 0713[理学-生态学] 

基　　金：National Natural Science Foundation of China (NO.31572072 31321004) 

主　　题：Acyrthosiphonpisum electroantennogram heterologous expression odorant receptor 

摘      要：The sensitive olfactory system is necessary for survival of *** receptors (ORs)are located on the dendrites of olfactory receptor neurons and play a critical role in odor *** ORs are functionally analyzed via heterologous expression in a Xenopus oocyte system using a two-electrode voltage-clamp (TEVC)electrophysiological ***,we have identified a novel OR in the pea aphid,Acyrthosiphon pisum,then we cloned and named it *** analyzed the ApisOR4 tissue expression patterns and found expression only in antennae *** functional analysis using TEVC revealed that ApisOR4 is broadly tuned to eight volatiles,which elicit electrophysiological response in pea aphid *** study provides an initial functional analysis of aphid ORs and identifies candidate volatiles to be used in developing new strategies for aphid control.