Electron Tomography in Plant Cell Biology

作     者：ThomasJ.Haas MarisaS.Otegui 

作者机构：Department of BotanyUniversity of Wisconsin-MadisonMadisonWI 53706USA 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2007年第49卷第8期

页      面：1091-1099页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 

基　　金：Supported by US National Foundation grant MCB-0619736 to MSO 

主　　题：cell structure electron tomography high-pressure freezing/freeze substitution. 

摘      要：This review focuses on the contribution of electron tomography-based techniques to our understanding of cellular processes in plant cells. Electron microscopy techniques have evolved to provide better three-dimensional resolution and improved preservation of the subcellular components. In particular, the combination of cryofixation/freeze substitution and electron tomography have allowed plant cell biologists to image organelles and macromolecular complexes in their native cellular context with unprecedented three-dimensional resolution （4-7 nm）. Until now, electron tomography has been applied in plant cell biology for the study of cytokinesis, Golgi structure and trafficking, formation of plant endosome/prevacuolar compartments, and organization of photosynthetic membranes. We discuss in this review the new insights that these tomographic studies have brought to the plant biology field.