Increased expression of intercellular adhesion molecule-1 in mouse focal cerebral ischemia model

作     者：葛海良 

作者机构：Institute of Shanghai Immunology Shanghai Second Medical University Shanghai 200025 China Institute of Shanghai Immunology Shanghai Second Medical University 280 South Chongqing Road Shanghai 200025 China Department of Surgery (Neurosurgery) University of Michigan Ann Arbor MI 48109 United States 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2000年第113卷第1期

页      面：75-79页

核心收录：

学科分类：1002[医学-临床医学] 10[医学] 

主　　题：Animals Blotting, Western Brain Ischemia Immunohistochemistry Intercellular Adhesion Molecule-1 Male Mice 

摘      要：OBJECTIVE: To quantitatively measure the temporal profiles of intercellular adhesion molecule-1 (ICAM-1) protein in mouse brain after middle cerebral artery occlusion (MCAO). METHODS: Adult male CD-1 mice received 0, 3, 6, 12, 24, 48 and 72 hour(s) of permanent MCAO with an intraluminal suture technique. The degree and the extent of occlusion were determined using a laser Doppler flowmeter. ICAM-1 positive expression in ischemic regions was determined immunohistochemically and ICAM-1 protein was quantitatively measured using immunoprecipitation and Western blot analysis. RESULTS: After MCAO, surface cerebral blood flow (CBF) in the ischemic hemisphere decreased to 9%-15% of the baseline in each time point of 7 to 8 animals. There were no significant differences in CBF measurement during occlusion between groups. Immunohistochemistry showed that ICAM-1 positive microvascular endothelial cells were observed both in the ischemic core and in the perifocal region. There was a tendency for increasing expression of ICAM-1 positive microvascular endothelial cells from the ischemic core to the ischemic margin. Western blot analysis showed that ICAM-1 expression in the ischemic hemisphere began to increase 3 h after MCAO, peaked at 6 h to 12 h, and persisted to 72 h. CONCLUSIONS: ICAM-1 expression increases in mice with permanent MCAO because ICAM-1 can mediate leukocyte-endothelial adhesion and progression of leukocyte infiltration after permanent focal cerebral ischemia. ICAM-1 is one of the important factors participating in ischemic cerebral damage and pathogenesis of stroke.