A proteomics study to explore the role of adsorbed serum proteins for PC12 cell adhesion and growth on chitosan and collagen/chitosan surfaces
作者机构：State Key Laboratory of BioelectronicsSchool of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096P.R.China SQ Medical Device Co.Ltd.Nanjing 210008P.R.China
出 版 物：《Regenerative Biomaterials》 (再生生物材料（英文版）)
年 卷 期：2018年第5卷第5期
页 面：261-273页
核心收录：
学科分类：081704[工学-应用化学] 07[理学] 070304[理学-物理化学(含∶化学物理)] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学]
基 金：supported by National Natural Science Foundation of China(31271012,31600816) the Natural Science Foundation of Jiangsu Province(BK20150599)
主 题：chitosan and collagen/chitosan film cell adhesion and growth protein adsorption proteomics/bioinformatics
摘 要：The aim of this article is to apply proteomics in the comparison of the molecular mechanisms of PC12 cell adhesion and growth mediated by the adsorbed serum proteins on the surfaces of chitosan and collagen/chitosan ***,the chitosan and the collagen/chitosan films were prepared by spin coating;and their surface morphologies were characterized by scanning electron microscopy,X-ray energy dispersive spectroscopy,contact angle measurement and Fourier transform infrared ***,cell proliferation experiments on two materials were performed and the dynamic curves of protein adsorption on their surfaces were ***,proteomics and bioinformatics were used to analyze and compare the adsorbed serum proteins on the surfaces of two biomaterials;and their effects on cell adhesion were *** results showed that the optimum concentration of chitosan film was 2%w/*** compared with chitosan film,collagen/chitosan film promoted the growth and proliferation of PC12 cells more *** the dynamic curves showed no significant difference in the total amount of the adsorbed proteins on both surfaces,proteomics and bioinformatics analyses revealed a difference in protein types:the chitosan surface adsorbed more vitronectin whereas collagen/chitosan surface adsorbed more fibronectin 1 and contained more cell surface receptor binding sites and more Leu-Asp-Val sequences in its surface structure;the collagen/chitosan surface were more conducive to promoting cell adhesion and growth.
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