Tea polyphenols increase X-ray repair cross-complementing protein 1 and apurinic/apyrimidinic endonuclease/redox factor-1 expression in the hippocampus of rats during cerebral ischemia/reperfusion injury

作     者：Zhi Wang Rongliang Xue Xi Lei Jianrui Lv Gang Wu Wei Li Li Xue Xiaoming Lei Hongxia Zhao Hui Gao Xin Wei 

作者机构：Department of Anesthesiology Second Affiliated Hospital of Medical College Xi'an Jiaotong University Xi'an 710004 Shaanxi Province China Department of Anesthesiology Stomatological Hospital Xi'an Jiaotong University Xi'an 710004 Shaanxi Province China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2012年第7卷第30期

页      面：2355-2361页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 071002[理学-动物学] 071003[理学-生理学] 

基　　金：supported by the National Natural Science Foundation of China  No. 30571790 

主　　题：global cerebral ischemia/reperfusion X-ray repair cross-complementing protein 1 apurinic/apyrimidinic endonuclease/redox factor-I tea polyphenols 

摘      要：Recent studies have shown that tea polyphenols can cross the blood-brain barrier, inhibit apoptosis and play a neuroprotective role against cerebral ischemia. Furthermore, tea polyphenols can decrease DNA damage caused by free radicals. We hypothesized that tea polyphenols repair DNA damage and inhibit neuronal apoptosis during global cerebral ischemia/reperfusion. To test this hypothesis, we employed a rat model of global cerebral ischemia/reperfusion. We demonstrated that intraperitoneal injection of tea polyphenols immediately after reperfusion significantly reduced apoptosis in the hippocampal CA1 region; this effect started 6 hours following reperfusion. Immunohistochemical staining showed that tea polyphenols could reverse the ischemia/reperfusion-induced reduction in the expression of DNA repair proteins, X-ray repair cross-complementing protein 1 and apudnic/apyrimidinic endonuclease/redox factor-1 starting at 2 hours. Both effects lasted at least 72 hours. These experimental findings suggest that tea polyphenols promote DNA damage repair and protect against apoptosis in the brain.