Neuronal differentiation effects of vascular endothelial factor on bone marrow stromal cells

作     者：Li Yi Qiaoyun Liu Jinling Han Jing Ye Fangting Zhang Guanghui Cui Zhuqing Zhou 

作者机构：Department of Neurology Peking University Shenzhen HospitaI Shenzhen 518036 Guangdong Province China Medical College of Shantou University Shantou 515041 Guangdong Province China Central Laboratory Peking University Shenzhen Hospital Shenzhen 518036 Guangdong Province China Laboratory of Male Reproductive Medicine Peking University Shenzhen Hospital Shenzhen 518036 Guangdong Province China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2010年第5卷第9期

页      面：657-661页

核心收录：

学科分类：10[医学] 

基　　金：the Science and Technology Research Program of Shenzhen No. 200802005 

主　　题：vascular endothelial growth factor bone marrow stromal cells neuronal-like cells cell differentiation nerve injury neural regeneration 

摘      要：BACKGROUND：Studies have demonstrated that bone marrow stromal cells （BMSCs） undergo neuronal differentiation under certain in vitro ***,very few inducers of BMSC differentiation have been used in clinical *** effects of vascular endothelial growth factor （VEGF） on in vitro neuronal differentiation of BMSCs remain poorly ***：To investigate the effect of VEGF on neuronal differentiation of BMSCs in vitro,and to determine the best VEGF concentration for experimental ***,TIME AND SETTING：In vitro comparative study was performed at the Central Laboratory and Laboratory of Male Reproductive Medicine,Shenzhen Hospital of Peking University from October 2008 to August ***：Recombinant human VEGF165 was purchased from Peprotech Asia,Rehovot,***-specific enolase （NSE） was purchased from Beijing Biosynthesis Biotechnology,***：BMSCs were harvested from adult Sprague Dawley *** passaged cells were pre-induced with 10 ng/mL basic fibroblast growth factor for 24 hours,followed by differentiation induction with 0,5,10,and 20 ng/mL VEGF,*** OUTCOME MEASURES：Morphological changes in BMSCs prior to and following VEGF *** of NSE following induction was determined by ***：Shrunken,round cells,with a strong refraction and thin bipolar or multipolar primary and secondary branches were observed 3 days after induction with 5,10,and 20 ng/mL ***,these changes were not observed in the control *** 10 days after induction,the number of NSE-positive cells was greatest in the 10 ng/mL VEGF-treated group （P〈 0.05）.The number of NSE-positive cells was least in the control group at 3 and 10 days post-induction （P〈 0.05）.Moreover,the number of NSE-positive cells was greater at 10 days compared with at 3 days after induction （P〈 0.05）.CONCLUSION：Of the VEGF concentrations tested,10 ng/mL induced the greatest number of neuronal-like cells in vitr