Simultaneous determination of three classes of antibiotics in the suspended solids of swine wastewater by ultrasonic extraction,solid-phase extraction and liquid chromatography-mass spectrometry

作     者：Xun Pan Zhimin Qiang Weiwei Ben Meixue Chen 

作者机构：Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2011年第23卷第10期

页      面：1729-1737页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0905[农学-畜牧学] 09[农学] 070302[理学-分析化学] 0703[理学-化学] 

基　　金：supported by the National Natural Science Foundation of China (No. 50878206, 50921064) the Ministry of Science and Technology of China (No. 2007AA06Z344,2009BAC57B02, 2009AA063901) 

主　　题：antibiotics swine wastewater suspended solids ultrasonic extraction solid-phase extraction mass spectrometry 

摘      要：This work describes a systematic approach to the development of a method for simultaneous determination of three classes of veterinary antibiotics in the suspended solids （SS） of swine wastewater, including five sulfonamides, three tetracyclines and one macrolide （tiamulin）. The entire procedures for sample pretreatment, ultrasonic extraction （USE）, solid-phase extraction （SPE）, and liquid chromatography-mass spectrometry （LC-MS） quantification were examined and optimized. The recovery efficiencies were found to be 76%-104% for sulfonamides, 81%-112% for tetracyclines, and 51%--64% for tiamulin at three spiking levels. The intra-day and inter-day precisions, as expressed by the relative standard deviation （RSD）, were below 17%. The method detection limits （MDLs） were between 0.14 and 7.14 μg/kg, depending on a specific antibiotic studied. The developed method was applied to field samples collected from three concentrated swine feeding plants located in Beijing, Shanghai and Shandong province of China. All the investigated antibiotics were detected in both SS and liquid phase of swine wastewater, with partition coefficients （logKd） ranging from 0.49 to 2.30. This study demonstrates that the SS can not be ignored when determining the concentrations of antibiotics in swine wastewater.