Bioproduction of D-psicose using permeabilized cells of newly isolated Rhodobacter sphaeroides SK011

作     者：Longtao ZHANG Bo JIANG Wanmeng MU Tao ZHANG 

作者机构：State Key Laboratory of Food Science and TechnologyJiangnan UniversityWuxi 214122China Institute of Animal Husbandry and Veterinary MedicineFujian Academy of Agricultural SciencesFuzhou 350013China 

出 版 物：《Frontiers of Chemical Science and Engineering》 (化学科学与工程前沿（英文版）)

年 卷 期：2009年第3卷第4期

页      面：393-398页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported financially by the National High Technology Research and Development Program of China(Grant No.2006AA10Z334) the Research Program of Sate Key Laboratory of Food Science and Technology,Jiangnan University(SKLF-MB-200804 and SKLF-TS-200805) 

主　　题：bioproduction D-psicose permeabilized cells Rhodobacter sphaeroides 

摘      要：D-psicose exits in an extremely small amount in nature and is difficult to be chemically *** three bacteria have been used in the biotransformation of D-psicose from *** this paper,another bacterium which could convert D-fructose to D-psicose was isolated and identified as Rhodobacter *** process parameters of D-psicose production using permeabilized cells of Rhodobacter sphaeroides SK011 were optimized,including the permeabilization procedure:0.1%(w/v)CTAB,10 min,and reaction conditions:cell concentration,30 g dry cell wt/L;concentration of substrate,50 g/L;40℃,pH 9.0;reaction time,8 *** the optimized conditions,the permeabilized cells produced approximately 6.5 g/L D-psicose with a Dpsicose productivity of 0.82 g·L^(-1)·h^(-1).This is the first report of bioproduction of D-psicose using permeabilized cells of Rhodobacter sphaeroides.