Inhibition of 12-lipoxygenase reduces proliferation and induces apoptosis of hepatocellular carcinoma cells in vitro and in vivo

作     者：Xi-Ming Xu,Guang-Jin Yuan,Jun-Jian Deng,Hong-Ting Guo,Miao Xiang,Fang Yang,Wei Ge and Shi-You Chen Cancer Center, Renmin Hospital of Wuhan University, Wuhan 430060, China Cancer Center, the 82nd Hospital of the Chinese PLA, Huai’an 223001, China Department of Physiology, Medical College of Wuhan University, Wuhan 430071, China Department of Physiology & Pharmacology, University of Georgia, Athens, GA 30602, USA 

作者机构：Cancer Center Renmin Hospital of Wuhan University Wuhan 430060 China Cancer Center the 82nd Hospital of the Chinese PLA Huai'an 223001 China Department of Physiology Medical College of Wuhan University Wuhan 430071 China Department of Physiology & Pharmacology University of Georgia Athens GA 30602 USA 

出 版 物：《Hepatobiliary & Pancreatic Diseases International》 (国际肝胆胰疾病杂志（英文版）)

年 卷 期：2012年第11卷第2期

页      面：193-202页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by grants from the National Natural Science Foundation of China(81000998) the Natural Science Foundation of Hubei Province,China(2007ABA248) the Foundation of the Ministry of Education of China for New Teachers(20090141120003) 

主　　题：hepatocellular carcinoma 12-lipoxygenase proliferation apoptosis 

摘      要：BACKGROUND:12-lipoxygenase(12-LOX) has been reported to be an important gene in cancer cell proliferation and survival,and tumor ***,its role in hepatocellular carcinoma(HCC) cells remains ***:Expression of 12-LOX was assessed in a diethylnitrosamine-induced rat HCC model,and in SMMC-7721,HepG2 and L-02 cells using immunohistochemical staining and reverse transcriptase-polymerase chain reaction(RT-PCR).GST-π and Ki-67 were determined in vivo by immunohistochemical *** was evaluated by TUNEL *** viability and apoptosis were determined by MTT assay and flow cytometry,***-related proteins in SMMC-7721 and HepG2 cells were detected by Western ***:Immunohistochemical staining and RT-PCR showed that 12-LOX was over-expressed in rat HCC and two HCC cell lines,while the expression was inhibited by baicalein,a specific inhibitor of *** inhibited cell proliferation and induced apoptosis in rat HCC and both cell lines in a dose-and time-dependent *** in vivo study demonstrated that baicalein also reduced neoplastic ***,baicalein reduced Bcl-2 protein expression coupled with a slight increase of the expression of Bax and activation of ***,baicalein inhibited the activation of ERK-1/2(phosphorylated).Interestingly,the effects of baicalein were reversed by 12(S)-HETE,a metabolite of ***:Inhibition of 12-LOX leads to reduced numbers of HCC cells,partially caused by increased apoptosis.12-LOX may be a potential molecular target for HCC prevention and treatment.