Histopathology of renal asphyxia in newborn piglets: Individual susceptibility to tubular changes

作     者：Clara Gerosa Nicoletta Iacovidou Ioanna Argyri Daniela Fanni Apostolos Papalois Filippia Aroni Gavino Faa Theodoros Xanthos Vassilios Fanos 

作者机构：Department of PathologyUniversity of Cagliari National and Kapodistrian University of Athens ELPEN Research-Experimental Center Laboratory of Experimental Surgery and Surgical ResearchUniversity of Athens Department of NeonatologyUniversity of Cagliari 

出 版 物：《World Journal of Nephrology》 (世界肾病学杂志（英文版）)

年 卷 期：2015年第4卷第2期

页      面：313-318页

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：Asphyxia Kidney Tubular eosinophilia Tubular dilatation Vacuolization Sloughing Apoptosis Brush border fragmentation 

摘      要：AIM： To analyze the effects on the kidney of hypoxia-reoxygenation in an experimental model of normocapnic ***： To this end, 40 newborn Landrace/Large-White piglets aged 1-4 d were studied in this work. Hypoxia was induced by decreasing the inspired fiO2 to 0.06-0.08. Animals were resuscitated with different fO2 and subdivided into 4 groups： group 1, 2, 3 and 4 received 18%, 21%, 40% and 100% O2 respectively. Macroscopic examination was carried out to evidence possible pathological features. Tissue sample were obtained from both kidneys. Four or fve micron paraffn sections were stained with H-E and PAS stain and examined under an optical ***： Pathological changes, mainly affecting tubular cells, were observed in the vast majority of kidneys of asphyxiated piglets. The most frequent tubular changes were： tubular casts （95%）, tubulardilatation （87.5%）, tubular vacuolization （70%）, tubular eosinophilia （52.5%）, sloughing （50%）, fragmentation of the brush border （50%）, oedema （32.5%）, apoptosis （15%） and glomerular changes （meningeal cell pro-liferation, capsular adhesion between the flocculus and Bowman’s capsule, glomerulosclerosis and fbrous or cellular crescents associated with collapse of the glomerular tuft）. Statistical analysis was carried out on changes observed when the animals were allocated in the 4 groups （χ2-test 0.05）. The statistical analysis showed no evidence of differences regarding kidney lesions among the animals ***： Our data show that renal pathology in newborn piglets is characterized by interindividual variability to hypoxia and is not associated with oxygenGerosa C et al . Individual susceptibility in renal asphyxiaconcentration.