Microbial community structure in different wastewater treatment processes characterized by single-strand conformation polymorphism (SSCP) technique

作     者：Yangguo ZHAO Aijie WANG Nanqi REN Yan ZHAO 

作者机构：School of Municipal and Environmental EngineeringHarbin Institute of TechnologyHarbin 150090China College of Environmental Science and EngineeringOcean University of ChinaQingdao 266100China 

出 版 物：《Frontiers of Environmental Science & Engineering》 (环境科学与工程前沿（英文）)

年 卷 期：2008年第2卷第1期

页      面：116-121页

核心收录：

学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 08[工学] 

基　　金：This work was supported by the National Natural Science Foundation of China(Grant Nos.:50208006,30470054 and 50678049) China Postdoctoral Science Foundation(Grant No.:20070410266) 

主　　题：wastewater treatment process microbial community structure community diversity single-strand conformation polymorphism(SSCP) 

摘      要：In order to investigate microbial community structures in different wastewater treatment processes and understand the relationship between the structures and the status of processes,the microbial community diversity,variety and distribution in five wastewater treatment pro cesses were studied by a culture-independent genetic fingerprinting technique single-strand conformation poly-morphism(SSCP).The five processes included denitrifying and phosphate-removal system(diminished N),Chinese traditional medicine wastewater treatment system(P),beer wastewater treatment system(W),fermentative biohydrogen-producing system(H),and sulfate-reduction system(S).The results indicated that the microbial community profiles in the wastewater bioreactors with the uniform status were very *** diversity of microbial populations was correlated with the complexity of organic contaminants in *** traditional medicine wastewater contained more complex organic components;hence,the population diversity was higher than that of simple nutrient bioreactors fed with molasses *** with the strain bands in a simulated community,the relative proportion of some functional microbial populations in bioreactors was not *** biohydrogen producer Ethanoligenens harbinense in the better condition bioreactor had only a 5% band density,and the Desulfovibrio *** the sulfate-reducing bioreactor had less than 1.5%band *** SSCP profiles could identify the difference in microbial community structures in wastewater treatment processes,monitor some of the functional microbes in these processes,and consequently provide useful guidance for improving their efficiency.