Changes in myocardial collagen content before and after left ventricular assist device application in dilated cardiomyopathy

作     者：梁红 Johannes Müller 翁渝国 Gerd Wallukat 付平 林汉生 Sabina Bartel Christoph Knosalla Reinhard Pregla Roland Hetzer 

作者机构：Department of Cardiovascular and Thoracic Surgery Medical College of Jinan University Deutsches Herzzentrum Berlin Max-Delbrück Centre for Molecular Medicine Department of Cardiovascular Surgery Institute of Cardiovascular Diseases Tongji Medical University Department of Medical Statistics Medical College of Jinan University 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2004年第117卷第3期

页      面：401-407页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：Heart-Assist Devices Adult Cardiomyopathy, Dilated Collagen Comparative Study Female Heart Transplantation Humans Hydroxyproline Male Middle Aged Myocardium Procollagen Prognosis 

摘      要：BACKGROUND: The purposes of this study were to confirm the changes in myocardial collagen level after left ventricular assist device (LVAD) support in dilated cardiomyopathy (DCM), find the relation between these changes and prognosis, and test a practical method to assess the level of myocardial collagen. METHODS: Left ventricular samples were collected from DCM patients with different prognosis (transplanted group n = 8, weaning group n = 10) at the time when the LVADs were implanted and again during cardiac transplantation (n = 8). The level of neutral salt soluble collagen (NSC) and acid soluble collagen (ASC) was measured by Sircol collagen assay, and that of total collagen and insoluble collagen (ISC) by quantification of hydroxyproline (Hyp). Serum samples were collected from a portion of these patients (transplanted group, n = 6; weaning group n = 7) at the time the LVADs were implanted, 1 month after implantation and on explantation. Circulating concentration of carboxy-terminal propeptide of type I procollagen (P I CP), amino-terminal propeptide of type I procollagen (P I NP), amino-terminal propeptide of type III procollagen (P III NP) and type I collagen telopeptide (I CTP) were measured by the equilibrium type radioimmunoassay. RESULTS: Before LVAD implantation the level of NSC and ISC in the weaning group was higher but ASC in the transplanted group was lower than in the controls (P