In Vivo Therapeutic Silencing of Hypoxia-Inducible Factor 1 Alpha(HIF-1)Using Single-Walled Carbon Nanotubes Noncovalently Coated with siRNA

作     者：Geoffrey Bartholomeusz Paul Cherukuri John Kingston Laurent Cognet Robert Lemos Jr Tonya KLeeuw Laura Gumbiner-Russo RBruce Weisman Garth Powis 

作者机构：Department of Experimental TherapeuticsUniversity of Texas M.D.Anderson Cancer Center1515 Holcombe Blvd.HoustonTX 77030USA Department of ChemistryCenter for Biological and Environmental Nanotechnologyand Institute of Biosciences and BioengineeringRice University6100 Main StreetHoustonTX 77005USA Centre de Physique Moléculaire Optique et HertzienneUniversitéBordeaux 1 and CNRS351 cours de la Libération33405 Talence CedexFrance 

出 版 物：《Nano Research》 (纳米研究（英文版）)

年 卷 期：2009年第2卷第4期

页      面：279-291页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：the M.D.Anderson Cancer Center from the NIH(CA-77204 and CA-109552) to Rice University from the Welch Foundation(C-0807) the NSF Center for Biological and Environmental Nanotechnology(EEC-0647452) the Alliance for NanoHealth(NASA JSC-NNJ06HC25G) 

主　　题：siRNA single-walled carbon nanotubes anti-cancer therapy in vivo delivery agent 

摘      要：A new approach is described for delivering small interfering RNA(siRNA)into cancer cells by noncovalently complexing unmodifi ed siRNA with pristine single-walled carbon nanotubes(SWCNTs).The complexes were prepared by simple sonication of pristine SWCNTs in a solution of siRNA,which then served both as the cargo and as the suspending agent for the *** complexes containing siRNA targeted to hypoxia-inducible factor 1 alpha(HIF-1)were added to cells growing in serum containing culture media,there was strong specific inhibition of cellular HIF-1 *** ability to obtain a biological response to SWCNT/siRNA complexes was seen in a wide variety of cancer cell ***,intratumoral administration of SWCNT-HIF-1 siRNA complexes in mice bearing MiaPaCa-2/HRE tumors signifi cantly inhibited the activity of tumor *** elevated levels of HIF-1 are found in many human cancers and are associated with resistance to therapy and decreased patient survival,these results imply that SWCNT/siRNA complexes may have value as therapeutic agents.