Real-time mechanical characterization of DNA degradation under therapeutic X-rays and its theoretical modeling

作     者：Grégoire Perret Thomas Lacornerie Fabio Manca Stefano Giordano Momoko Kumemura Nicolas Lafitte Laurent Jalabert Mehmet C.Tarhan Eric F.Lartigau Fabrizio Cleri Hiroyuki Fujita Dominique Collard 

作者机构：LIMMS/CNRS-IIS UMI 2820Institute of Industrial ScienceThe University of Tokyo4-6-1 Komaba Meguro KuTokyo 153-8505Japan IEMNUMR8520CNRSAvenue PoincaréCitéScientifiqueBP 60069Villeneuve d’AscqCedex 59652France CNRS/IIS/COL/Lille 1 SMMiL-E projectCNRS Délégation Nord-Pas de Calais et Picardie2 rue de CanonniersLilleCedex 59046France Centre Oscar LambretUniversitéde LilleDépartement Universitaire de RadiothérapieCentre Oscar LambretLille 59000France Institute of Industrial ScienceThe University of Tokyo4-6-1 KomabaMeguro-kuTokyo 153-8505Japan 

出 版 物：《Microsystems & Nanoengineering》 (微系统与纳米工程（英文）)

年 卷 期：2016年第2卷第1期

页      面：9-17页

核心收录：

学科分类：0808[工学-电气工程] 1002[医学-临床医学] 0809[工学-电子科学与技术（可授工学、理学学位）] 0817[工学-化学工程与技术] 0807[工学-动力工程及工程热物理] 0805[工学-材料科学与工程（可授工学、理学学位）] 0802[工学-机械工程] 100214[医学-肿瘤学] 0811[工学-控制科学与工程] 0702[理学-物理学] 10[医学] 

基　　金：G.P.received a Doctoral Scholarship from the Institut National du Cancer and additional financial support provided by CNRS 

主　　题：biomechanical measurements DNA damage microfluidic radiotherapy real-time Silicon Nanotweezers 

摘      要：The killing of tumor cells by ionizing radiation beams in cancer radiotherapy is currently based on a rather empirical understanding of the basic mechanisms and effectiveness of DNA damage by *** contrast,the mechanical behaviour of DNA encompassing sequence sensitivity and elastic transitions to plastic responses is much better understood.A novel approach is proposed here based on a micromechanical Silicon Nanotweezers *** instrument allows the detailed biomechanical characterization of a DNA bundle exposed to an ionizing radiation beam delivered here by a therapeutic linear particle accelerator(LINAC).The micromechanical device endures the harsh environment of radiation beams and still retains molecular-level detection *** this study,the first real-time observation of DNA damage by ionizing radiation is *** DNA bundle degradation is detected by the micromechanical device as a reduction of the bundle stiffness,and a theoretical model provides an interpretation of the *** first real-time observations pave the way for both fundamental and clinical studies of DNA degradation mechanisms under ionizing radiation for improved tumor treatment.